DNA染色剂Gold View 是不是吖啶橙(AO)?
做分子生物学的都知道,EB是最长用和灵敏度很高的DNA染色剂,但是其具有极高的诱变作用,因而近年来,一种EB替代品Gold View就出现了,号称最安全的DNA染色剂。
但是,今天从网上忽然找到这样一则: 网址:https://www.ope-tech.com/doc/GelRed.asp
Mass Spectrometry Analysis of GoldView:
Conclusion: The effective ingredient in GoldView is acridine orange
(extremely cheap from Aldrich or Sigma at ~100g/$140!)
Acridine orange is a known gel stain since 1970s
(“Analysis of single- and double-stranded nucleic acids on polyacrylamide
and agarose gels by using glyoxal and acridine orange”.
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.
Acridine orange is a highly toxic and known mutagen!
Selected list of papers on acridine orange toxicity: Web, RB. Mutat Res. 1984 Jul;137(1):1-6.
Wugmeister, M. Yale J Biol Med. 1983 Jan-Feb;56(1):9-13.
Martin, SE. Mutat Res. 1981 Jun;82(1):41-6. Ashad, R. Letters in Applied Microbiology 2006,42:94.
问题出来了,和吖啶橙一样的有效成分,会是最安全的DNA染色剂?从质谱来看,最多是修饰基团不一样啊??
突然想到一个邪恶的念头,中国的试剂生产商,你们有没有拿我们当小白鼠?