【试剂盒信息】————————————————————————————————
ARY029：Proteome Profiler Human NFkB Pathway Array Kit，
可用于检测NFKB通路41种不同蛋白的表达水平，其中有4个是丝氨酸和络氨酸的磷酸化抗体。
【试剂盒背景信息】——————————————————————————————
The NFκB signaling pathway plays a central role in many physiological and pathological processes. Dysregulation of NFκB activity is associated with inflammatory disorders, autoimmune and metabolic diseases, as well as cancer. Profiling NFκB signaling pathway proteins may help to better understand their underlying mechanisms. The Human NFκB Pathway Array is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of 41 proteins and 4 serine or tyrosine phosphorylation sites involved in NFκB signal transduction without performing numerous immunoprecipitations and Western blots. Each capture antibody was carefully selected using cell lysates prepared from cell lines known to express the target protein.
【试剂盒组分】————————————————————————————————
√ 4块硝酸纤维素膜
√ 点在膜上的捕获抗体
√ Array Buffers √ 25×的洗涤缓冲液
√ ECL底物
√ 生物素或HRP标记的检测抗体 √ 4孔方孔板
√ 透明模板
√ 产品说明书
【试剂盒可检测的指标清单】——————————————————————————
Simultaneously detect the levels of these proteins in a single sample.
ASC IL-18 R alpha RelA/p65 (pS529)
BCL-10 IRAK1 c-Rel
CARD6 IRF5 SHARPIN
CD40/TNFRSF5 IRF8 SOCS-6
dAP1/BIRC2 JNK1/2 STAT1p91
dAP2/BIRC3 JNK2 STAT1 (pY701)
FADD/MORT1 LTBR/TNFRSF3 STAT2
Fas/TNFRSF6/CD95 Metadherin/AEG-1 STAT2 (pY689)
IκBα MYD88 STING/TMEM173
IκBε NFκB1 TLR2
IKK1/IKKα/CHUK NFκB2 TNF RI/TNFRSF1A
IKK2/IKKβ NGF R/TNFRSF16 TNF RII/TNFRSF1B
IKKυ/NEMO P53 TRAF2
IL-1 RI P53 (pS46) TRAIL R1/DR4
IL-17 RA RelA/p65 TRAIL R2/DR5
【Data Examples】———————————————————————————————
HCT-116 Untreated vs. Epoxomicin Treated
HCT-116 human colorectal carcinoma cells were untreated or treated with 1 µM Epoxomicin (Boston Biochem, Catalog # I-110) for 6 hours (500 µg lysate, 5 minute exposure). RS = Reference Spots.
Daudi Untreated vs. IFN-alpha 2A Treated
Daudi human Burkitt’s lymphoma cells were untreated or treated with 500 U/mL IFN-alpha 2A (R&D Systems, Catalog # 11100-1) for five minutes (1 mg lysate, 10 minute exposure).
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