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瞬间吸收成像系统可追踪血液中碳纳米管

2011/12/08 来源:科学网
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美国普渡大学的研究人员发明了一种追踪活体细胞和血液中碳纳米管的成像新技术,使得纳米管在生物医学研究和临床医学的应用趋于完美。

美国普渡大学的研究人员发明了一种追踪活体细胞和血液中碳纳米管的成像新技术,使得纳米管在生物医学研究和临床医学的应用趋于完美。相关研究论文在线发表于12月4日的《自然—纳米技术》杂志上。

碳纳米管

纳米管目前有两种,它们在药物输送和癌症研究成像中具有潜在应用价值;然而至今没有一种技术可以在活体细胞和血液中观察到它们。此次发明的技术叫做“瞬间吸收成像系统”,利用脉冲近红外激光将能量送入纳米管,之后再由第二束激光探测。该技术不需要用染料来标记纳米管,使得其在科研和医药应用上具潜在的实用价值。此外,科学家们通过使信号由不同的“通道”经过红细胞和纳米管,从而消除勒红细胞的背景干扰。

该研究领导者、华人科学家Ji-Xin Cheng表示,该技术可以实时观测纳米管在血液中的循环,可以为研究者提供相关信息,从而了解如何完美地在研究和临床上应用纳米管。


Label-free imaging of semiconducting and metallic carbon nanotubes in cells and mice using transient absorption microscopy

Ling Tong, Yuxiang Liu, Bridget D. Dolash, Yookyung Jung,4Mikhail N. Slipchenko, Donald E. Bergstrom,  & Ji-Xin Cheng

As interest in the potential biomedical applications of carbon nanotubes increases, there is a need for methods that can image nanotubes in live cells, tissues and animals. Although techniques such as Raman photoacoustic and near-infrared photoluminescence imaging have been used to visualize nanotubes in biological environments, these techniques are limited because nanotubes provide only weak photoluminescence and low Raman scattering and it remains difficult to image both semiconducting and metallic nanotubes at the same time. Here, we show that transient absorption microscopy offers a label-free method to image both semiconducting and metallic single-walled carbon nanotubes in vitro and in vivo, in real time, with submicrometre resolution. By using appropriate near-infrared excitation wavelengths, we detect strong transient absorption signals with opposite phases from semiconducting and metallic nanotubes. Our method separates background signals generated by red blood cells and this allows us to follow the movement of both types of nanotubes inside cells and in the blood circulation and organs of mice without any significant damaging effects.

文献链接http://www.nature.com/nnano/journal/vaop/ncurrent/full/nnano.2011.210.html#affil-auth

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